President Donald Trump stirred the hearts of the University of Alabama graduates when he re-affirmed his promise to "keep men out of women's sports."

During a commencement speech at the university's graduation ceremony on Thursday night, Trump gave a shutout to the school's SEC champion women's track and field team, before igniting a raucous applause by "vowing to defend women's sports."

"As long as I'm president, we will always protect women's sports, men will not play in women's sports!" Trump said, before the crowd erupted in cheers, for its loudest and longest applause of the night. 

"No way! They say its an 80-20 issue, no, it's a 97-3 issue, I think," Trump said. "No, men will not be playing in women's sports. I said that and I classified it with a very powerful executive order as you know, it's done." 

CLICK HERE FOR MORE SPORTS COVERAGE ON FOXNEWS.COM

Later in the speech Trump circled back to the subject later in the speech, mocking the Democrats for allowing trans athletes in women's sports and the trans athletes themselves in a lengthy rant. 

During this section of the speech, Trump also discussed the Paris Olympics women's boxing competitions, which included two gold medalists who were previously disqualified from international competitions for failing gender eligibility tests. However, neither boxer, Algeria's Imane Khelif and Taiwan's Lin Yu-ting, identifies as transgender. 

"They had a great champion a female boxer, and after one punch she walked back to the corner and said ‘I can’t get hit like that, I've never been hit like that before," Trump said. 

At one point, Trump did a physical impersonation of a female weight lifter and a trans weight lifter, and reenacted a scenario where the female loses a competition to a trans opponent. 

Then Trump took aim at transgender swimmers, telling a story of a swimmer who he joked was "windburned" by a trans opponent. 

"One young lady, she was going to set the record, she fought all her life to set the record," Trump said. "Then she looks to the right and she sees the same thing but there's a person next to her who's a giant… that was a person that transitioned and he had the wingspan of Wilt ‘the stilt’ Chamberlain." 

Trump made similar references to the weightlifting and swimmer scenarios in June 2023 while speaking at the North Carolina Republican Party's convention in Greensboro. 

On Thursday, Trump also referenced female volleyball players who have been impacted by trans inclusion. 

"You look at all the volleyball players who have been hurt so badly that are hit at levels that they've never seen before," Trump said. 

One former University of Alabama women's volleyball player, Brooke Slusser, was thrust into a situation where she had to share a locker room and bedroom with a trans athlete when she transferred from the university to San Jose State University in 2023. 

There, after leaving Alabama for California, Slusser was thrust into a situation where she was made to share those spaces with trans teammate Blaire Fleming without even being told Fleming is a biological male, Slusser alleges in a lawsuit.

HOW TRANSGENDERISM IN SPORTS SHIFTED THE 2024 ELECTION AND IGNITED A NATIONAL COUNTERCULTURE

Slusser has since fled San Jose State University and returned home to Texas after facing alleged backlash and harassment in the aftermath of filing her lawsuit. 

Trump signed the "Keeping Men Out of Women's Sports" executive order on Feb. 5. One day later, the NCAA revised its gender eligibility police to restrict participation in the women's category to only biological females. However, the new policy has also come under criticism by some women's sports activists for not going far enough. 

Alabama as a state has had a law in place to prevent trans athletes in girls' sports in effect since 2021, and in 2023 it extended to include college students. Unlike other laws addressing the issue, Alabama's law also bars athletes assigned female at birth from participating in the boys' category unless there is no comparable girls' opportunity (such as football).

Trans inclusion in women and girls' sports emerged as a hot-button issue in Trump's 2024 election victory, as most Americans came to take the Republican's side on the topic. 

A national exit poll conducted by the Concerned Women for America (CWA) legislative action committee found that 70% of moderate voters saw the issue of "Donald Trump’s opposition to transgender boys and men playing girls and women’s sports and of transgender boys and men using girls and women’s bathrooms," as important to them. 

And 6% said it was the most important issue of all, while 44% said it was "very important."

The issue inspired a national counter-culture movement against Demorat policies that keep trans athletes in women's sports, heavily influenced by young college-educated women. And Biden’s 35-point lead among young women over Trump in 2020 shrunk to a 24-point lead for Harris this year, per an NBC News exit poll. 

A New York Times/Ipsos survey found the vast majority of Americans, including a majority of Democrats, don't think transgender athletes should be permitted to compete in women's sports. 

Of the 2,128 people who participated, 79% said biological males who identify as women should not be allowed to participate in women's sports. Of the 1,025 people who identified as Democrats or leaning Democrat, 67% said transgender athletes should not be allowed to compete with women. 

Nearly 70% of Americans say biological men should not be permitted to compete in women's sports, according to a Gallup poll last year.

In June 2024, a survey conducted by NORC at the University of Chicago asked respondents whether transgender athletes of both sexes should be permitted to participate in sports leagues that correspond to their preferred gender identity instead of their biological sex. In that survey, 65% answered that it should never or rarely be allowed. When those polled were asked specifically about adult transgender female athletes competing in women’s sports, 69% opposed it.

Follow Fox News Digital’s sports coverage on X, and subscribe to the Fox News Sports Huddle newsletter.

President Donald Trump's newly formed Title IX investigative team has been called upon in the state of Washington. 

Department of Education (DOE) Secretary Linda McMahon announced Wednesday the team will be launching an investigation into Washington Superintendent of Public Instruction Chris Reykdal's office. 

The team, a joint initiative by the DOE and the U.S. Department of Justice (DOJ), is taking action in response to Reykdal requiring schools to allow biologically male trans athletes on girls sports teams. 

"Multiple Washington state school districts have reported that OSPI is requiring school boards to adopt policies that allow males to participate in female sports and occupy female-only intimate facilities, thereby raising substantial Title IX concerns," the announcement said. 

CLICK HERE FOR MORE SPORTS COVERAGE ON FOXNEWS.COM

McMahon issued a warning to Reykdal and the rest of the Democrat-controlled state.

"Washington state appears to use its position of authority to coerce its districts into hiding ‘gender identity’ information from students’ parents and to adopt policies to covertly smuggle gender ideology into the classroom, confusing students and letting boys into girls sports, bathrooms and locker rooms," McMahon said.

"If true, these are clear violations of parental rights and female equality in athletics, which are protected by federal laws that will be enforced by the Trump administration." 

Reykdal provided a statement to Fox News Digital and did not indicate his office would cooperate with the federal investigation. 

"My job as the leader of this constitutional office is to communicate, uphold and enforce the law," Reykdal said. "My office will enforce our current laws as we are required to do until Congress changes the law and/or federal courts invalidate Washington state’s laws. Unless, and until that happens, we will be following Washington state’s laws, not a president’s political leanings expressed through unlawful orders." 

Reykdal spoke in defense of transgender athletes in girls sports in an address Feb. 20, claiming it was "inaccurate" to say there are only two genders. 

"It is quite simply inaccurate to say, biologically, that there are only boys and there are only girls," Reykdal said on camera. "There's a continuum. There's a science to this. There are children who are born intersex. There are children whose hormones and whose chromosomes are not consistent with their sex at birth." 

STATE EDUCATION CHIEF SAYS IT'S ‘INACCURATE’ TO SAY THERE ARE ONLY TWO GENDERS, IN DEFENSE OF TRANS ATHLETES

Washington's high school athletes are allowed to compete based on their gender identity rather than their biological sex. Washington Interscholastic Activities Association (WIAA) policy states that each athlete will participate in programs "consistent with their gender identity or the gender most consistently expressed," and there are no medical or legal requirements. 

Bills that would prohibit transgender girls from participating in girls and women's sports have been introduced but not passed.

The Kennewick School Board filed a Title IX complaint with the DOE's Office of Civil Rights against Reykdal's office in late March over the issue. 

Kennewick School Board President Gabe Galbraith previously told Fox News the district is seeking federal intervention to ensure the order will eventually be honored.

SCHOOL BOARD PLEADS WITH TRUMP ADMIN TO FORCE STATE BAN ON TRANSGENDERS IN GIRLS SPORTS AS DEMOCRATS REFUSE

"There's boys participating in girls sports. There's boys in girls locker rooms. It's unfair. It's not safe, and we're asking the federal government to just step in and put an end to this and ensure that the state is following President Trump's statements," he said.

Other school districts have taken a stand against Reykdal since President Trump signed his "Keeping Men Out of Women's Sports" executive order Feb. 5. 

The Tumwater School District's board of directors voted later that month to ban trans girls from playing for girls sports teams. The resolution passed by a vote of 3-1 after a civil rights complaint was filed against the district over an incident involving a trans athlete in a girls basketball game. 

The complaint alleged that the Tumwater School District in Washington is investigating 15-year-old Frances Staudt for "misgendering" the opponent and violating the district's policies against bullying and harassment Feb. 7. 

According to the document, Staudt asked the school's principal and athletic director before the game whether the player was a biological male. The administrators then allegedly confirmed that they had been notified that the player was transgender but denied her pleas to have the player removed.

However, the DOE OCR announced an investigation into Tumwater School District just days later. 

Now, the Trump administration is going further up the chain of command in the state, and taking aim at the superintendent. 

In addition to reports that Washington schools are allowing trans athletes in girls sports, the DOE cited in its announcement Wednesday that La Center School District in La Center, Washington, received a letter of finding indicating Reykdal was requiring districts to enact policies, such as mandating that districts not notify parents of a change in their child’s "gender identity,"

Follow Fox News Digital’s sports coverage on X, and subscribe to the Fox News Sports Huddle newsletter.